Effect of nicotine on orthodontic tooth movement and bone remodeling in rats
이승희, 차정열, 최성환, 김백일, 차재국, 황충주,
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이승희 ( Lee Sung-Hee ) - Yonsei University College of Dentistry Department of Orthodontics
차정열 ( Cha Jung-Yul ) - Yonsei University College of Dentistry Department of Orthodontics
최성환 ( Choi Sung-Hwan ) - Yonsei University College of Dentistry Department of Orthodontics
김백일 ( Kim Baek-Il ) - Yonsei University College of Dentistry Department of Preventive Dentistry & Public Oral Health
차재국 ( Cha Jae-Kook ) - Yonsei University College of Dentistry Department of Periodontology
황충주 ( Hwang Chung-Ju ) - Yonsei University College of Dentistry Department of Orthodontics
Abstract
Objective: To quantitatively analyze the effect of nicotine on orthodontic tooth movement (OTM) and bone remodeling in rats using micro-computed tomography and tartrate-resistant acid phosphatase immunostaining.
Methods: Thirty-nine adult male Sprague?Dawley rats were randomized into three groups: group A, 0.5 mL normal saline (n = 9, 3 per 3, 7, and 14 days); group B, 0.83 mg/kg nicotine (n = 15, 5 per 3, 7, and 14 days); and group C, 1.67 mg/kg nicotine (n = 15, 5 per 3, 7, and 14 days). Each animal received daily intraperitoneal injections of nicotine/saline from the day of insertion of identical 30-g orthodontic force delivery systems. A 5-mm nickel-titanium closed-coil spring was applied between the left maxillary first molar (M1) and the two splinted incisors. The rate of OTM and volumetric bone changes were measured using micro-computed tomography. Osteoclasts were counted on the mesial alveolar bone surface of the distobuccal root of M1. Six dependent outcome variables, including the intermolar distance, bone volume fraction, bone mineral density, trabecular thickness, trabecular volume, and osteoclast number, were summarized using simple descriptive statistics. Nonparametric Kruskal?Wallis tests were used to evaluate differences among groups at 3, 7, and 14 days of OTM.
Results: All six dependent outcome variables showed no statistically significant among group-differences at 3, 7, and 14 days.
Conclusions: The findings of this study suggest that nicotine does not affect OTM and bone remodeling, although fluctuations during the different stages of OTM in the nicotine groups should be elucidated in further prospective studies.
키워드
Bone remodeling; Micro-computed tomography; Nicotine; Orthodontic tooth movement
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